| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pancreas/duodenum homeobox protein 1; PDX-1; Glucose-sensitive factor; GSF; Insulin promoter factor 1; IPF-1; Insulin upstream factor 1; IUF-1; Islet/duodenum homeobox-1; IDX-1; Somatostatin-transactivating factor 1; STF-1; PDX1; IPF1; STF1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PDX1 recombinant protein (Position: M1-E145). Human PDX1 shares 87.7% and 86.9% amino acid (aa) sequence identity with mouse and rat PDX1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes pancreas/duodenum homeobox protein 1 (gene PDX1) in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 30.8 kDa, and the supplier reports an observed band at about 40 kDa.
PDX1 (also IPF1) is a homeodomain transcription factor essential for pancreas development. It specifies early pancreatic progenitors and, in adult β-cells, drives expression of insulin and other genes needed for glucose-regulated insulin secretion, so it is a key marker in β-cell biology, diabetes research and stem cell differentiation.
| Target | Pancreas/duodenum homeobox protein 1 (gene PDX1; UniProt P52945, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 30.8 kDa |
| Observed MW | 40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human PC-3 cells, human HeLa cells, human 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with a confirmed signal (flow cytometry): Caco-2 cells.
Immunogen
Recombinant human PDX1 fragment (Met1–Glu145), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 87.7% and 86.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places pancreas/duodenum homeobox protein 1 in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
