| Field | Specification |
|---|---|
| Target | |
| Alternative names | Na; +; /H; exchange regulatory cofactor NHE-RF3; NHERF-3; CFTR-associated protein of 70 kDa; Na(+)/H(+) exchanger regulatory factor 3; Na/Pi cotransporter C-terminal-associated protein 1; NaPi-Cap1; PDZ domain-containing protein 1; Sodium-hydrogen exchanger regulatory factor 3; PDZK1; CAP70; NHERF3; PDZD1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PDZK1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23P73 is a rabbit monoclonal antibody that recognizes na(+)/H(+) exchange regulatory cofactor NHE-RF3 (gene PDZK1) in human samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 57.1 kDa, and the supplier reports an observed band at about 70 kDa.
PDZK1 (NHERF3) is a scaffold protein with four PDZ domains found at the apical membrane of epithelial cells in the kidney, intestine and liver. It binds transporters and receptors such as the HDL receptor SR-BI (SCARB1), CFTR and the anion exchanger SLC26A6, controlling their surface expression, and in the liver it is needed for normal SR-BI levels and cholesterol handling.
| Target | Na(+)/H(+) exchange regulatory cofactor NHE-RF3 (gene PDZK1; UniProt Q5T2W1, human) |
|---|---|
| Clone | 23P73 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 57.1 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): T47-D cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PDZK1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places na(+)/H(+) exchange regulatory cofactor NHE-RF3 in the membrane and cell membrane. Tissue expression noted by UniProt (human): Expression is limited to epithelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
