| Field | Specification |
|---|---|
| Target | |
| Alternative names | ETS translocation variant 4;Adenovirus E1A enhancer-binding protein;E1A-F;Polyomavirus enhancer activator 3 homolog;Protein PEA3;ETV4;E1AF, PEA3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Pea3, different from the related mouse sequence by seven amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets ETS translocation variant 4 (gene ETV4) in human and rat samples and is validated for Western blot. The predicted molecular weight is 53.9 kDa, and the supplier reports an observed band at about 68 kDa.
ETV4 (also called PEA3) is an ETS-family transcriptional activator of the PEA3 subgroup. It acts downstream of receptor tyrosine kinase and MAPK signaling, contributes to motor neuron positioning and axon branching during development, and is often overexpressed or rearranged in cancers such as prostate cancer.
| Target | ETS translocation variant 4 (gene ETV4; UniProt P43268, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 53.9 kDa |
| Observed MW | 68 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|
Samples with a confirmed band (WB): rat lung tissue, HeLa cells, A549 cells, SW620 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human pea3. It differs from the mouse sequence at seven residues.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places ETS translocation variant 4 in the nucleus. Tissue expression noted by UniProt (human): Expressed in keratinocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
