| Field | Specification |
|---|---|
| Target | |
| Alternative names | Paternally-expressed gene 3 protein; Zinc finger and SCAN domain-containing protein 24; PEG3; KIAA0287; ZSCAN24 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PEG3 recombinant protein (Position: M1-R1548). Human PEG3 shares 62.3% amino acid (aa) sequence identity with mouse PEG3. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets paternally-expressed gene 3 protein (gene PEG3) in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 180.8 kDa, and the supplier reports an observed band at about 200 kDa.
PEG3 is a paternally expressed, imprinted gene encoding a large zinc finger protein. It cooperates with SIAH1 in p53-dependent neuronal cell death and interacts with TRAF2 in TNF signaling, and mouse studies link it to maternal behavior and growth. It is studied in genomic imprinting and as a candidate tumor suppressor in glioma.
| Target | Paternally-expressed gene 3 protein (gene PEG3; UniProt Q9GZU2, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 180.8 kDa |
| Observed MW | 200 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): PC-3 cells.
Immunogen
Recombinant human PEG3 fragment (Met1–Arg1548), expressed in E. coli. Sequence identity with the mouse ortholog: 62.3%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places paternally-expressed gene 3 protein in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Brain, glial cells, astrocytes, embryo, placenta, testis, ovary and uterus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
