| Field | Specification |
|---|---|
| Target | |
| Alternative names | Perilipin-1; Lipid droplet-associated protein; PLIN1; PERI, PLIN; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Perilipin A, which shares 80% amino acid (aa) sequence identity with both mouse and rat Perilipin A. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes perilipin-1 (gene PLIN1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 56.0 kDa, and the supplier reports an observed band at about 56 kDa.
Perilipin-1 is the main coat protein of lipid droplets in adipocytes. In the basal state it shields stored triglycerides from lipases, and when PKA phosphorylates it after hormonal stimulation, it releases the lipase coactivator CGI-58 and allows lipolysis, making it a key regulator of fat storage and release.
| Target | Perilipin-1 (gene PLIN1; UniProt O60240, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 56.0 kDa |
| Observed MW | 56 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|
Samples with a confirmed band (WB): human HeLa cells, rat liver tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human perilipin A. Sequence identity with the mouse and rat orthologs: 80%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places perilipin-1 in the endoplasmic reticulum. Tissue expression noted by UniProt (human): Detected in adipocytes from white adipose tissue (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wang Shuai et al. (2024) SOX4 facilitates brown fat development and maintenance through EBF2-mediated thermogenic gene program in mice. Cell Death and Differentiation. 10.1038/s41418-024-01397-0
Zhizheng Fang et al. (2022) Phillyrin restores metabolic disorders in mice fed with high-fat diet through inhibition of interleukin-6-mediated basal lipolysis. Frontiers in Nutrition. 10.3389/fnut.2022.956218
Liao Naishun et al. (2019) Antioxidants inhibit cell senescence and preserve stemness of adipose tissue-derived stem cells by reducing ROS generation during long-term in vitro expansion. Stem Cell Research & Therapy. 10.1186/s13287-019-1404-9
