| Field | Specification |
|---|---|
| Target | |
| Alternative names | Perilipin-1;Lipid droplet-associated protein;PLIN1;PERI, PLIN; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Perilipin A |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAED-16 is a rabbit monoclonal antibody that recognizes perilipin-1 (gene PLIN1) in human and mouse samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 56.0 kDa, and the supplier reports an observed band at about 62 kDa.
Perilipin-1 is the main coat protein of lipid droplets in adipocytes. In the basal state it shields stored triglycerides from lipases, and when PKA phosphorylates it after hormonal stimulation, it releases the lipase coactivator CGI-58 and allows lipolysis, making it a key regulator of fat storage and release.
| Target | Perilipin-1 (gene PLIN1; UniProt O60240, human) |
|---|---|
| Clone | AAED-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 56.0 kDa |
| Observed MW | 62 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:3, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human perilipin A.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places perilipin-1 in the endoplasmic reticulum. Tissue expression noted by UniProt (human): Detected in adipocytes from white adipose tissue (at protein level).
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wang Shuai et al. (2024) SOX4 facilitates brown fat development and maintenance through EBF2-mediated thermogenic gene program in mice. Cell Death and Differentiation. 10.1038/s41418-024-01397-0
