| Field | Specification |
|---|---|
| Target | |
| Alternative names | Peroxiredoxin-2;1.11.1.15;Natural killer cell-enhancing factor B;NKEF-B;PRP;Thiol-specific antioxidant protein;TSA;Thioredoxin peroxidase 1;Thioredoxin-dependent peroxide reductase 1;PRDX2;NKEFB, TDPX1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Peroxiredoxin 2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IEO-16 is a rabbit monoclonal antibody that recognizes peroxiredoxin-2 (gene PRDX2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 21.9 kDa, and the supplier reports an observed band at about 22 kDa.
Peroxiredoxin 2 (PRDX2) is a cytosolic thiol peroxidase that removes hydrogen peroxide and organic hydroperoxides. It is especially abundant in red blood cells, where it guards against oxidative damage, and it also shapes hydrogen peroxide-dependent signaling, so it is a frequent marker in studies of oxidative stress and redox regulation.
| Target | Peroxiredoxin-2 (gene PRDX2; UniProt P32119, human) |
|---|---|
| Clone | IEO-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 21.9 kDa |
| Observed MW | 22 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human MCF-7 cells, human HeLa cells, human PC-3 cells, human RT4 cells, rat RH35 cells, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human peroxiredoxin 2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places peroxiredoxin-2 in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
