| Field | Specification |
|---|---|
| Target | |
| Alternative names | Thioredoxin-dependent peroxide reductase, mitochondrial;1.11.1.15;Antioxidant protein 1;AOP-1;HBC189;Peroxiredoxin III;Prx-III;Peroxiredoxin-3;Protein MER5 homolog;PRDX3;AOP1; |
| UniProt # | |
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| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Peroxiredoxin 3, different from the related mouse and rat sequences by three amino acids. |
| Molecular weight | |
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| Reconstitution | |
| Cellular localization | |
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| Form | Lyophilized |
| Storage | |
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Product Overview
This rabbit polyclonal antibody targets thioredoxin-dependent peroxide reductase, mitochondrial (gene PRDX3) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 27.7 kDa, and the supplier reports an observed band at about 26 kDa.
Peroxiredoxin 3 (PRDX3) is the mitochondrial member of the peroxiredoxin family, reducing hydrogen peroxide generated during respiration with the help of the mitochondrial thioredoxin system. It protects mitochondria from oxidative damage and has been linked to NF-κB regulation, making it relevant to studies of mitochondrial function, metabolism and cell survival.
| Target | Thioredoxin-dependent peroxide reductase, mitochondrial (gene PRDX3; UniProt P30048, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 27.7 kDa |
| Observed MW | 26 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human LN229 cells, rat C6 cells, human HepG2 cells, human A549 cells, human 293T cells, human Caco-2.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): rat intestine tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with a confirmed signal (flow cytometry): K562 cells.
Immunogen
Synthetic peptide from the C-terminal region of human peroxiredoxin 3. It differs from the mouse and rat sequences at three residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places thioredoxin-dependent peroxide reductase, mitochondrial in the mitochondrion, cytoplasm and early endosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Martínez-Rubio Dolores et al. (2022) Protein misfolding and clearance in the pathogenesis of a new infantile onset ataxia caused by mutations in PRDX3. Human Molecular Genetics. 10.1093/hmg/ddac146
