| Field | Specification |
|---|---|
| Target | |
| Alternative names | Peroxiredoxin-5, mitochondrial; Alu corepressor 1; Antioxidant enzyme B166; AOEB166; Liver tissue 2D-page spot 71B; PLP; Peroxiredoxin V; Prx-V; Peroxisomal antioxidant enzyme; TPx type VI; Thioredoxin peroxidase PMP20; Thioredoxin-dependent peroxiredoxin 5; PRDX5; ACR1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Peroxiredoxin 5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22P75 is a rabbit monoclonal antibody that recognizes peroxiredoxin-5, mitochondrial (gene PRDX5) in human, mouse and rat samples and is validated for Western blot, IHC, immunoprecipitation and flow cytometry. The predicted molecular weight is 22.1 kDa, and the supplier reports an observed band at about 17 kDa.
Peroxiredoxin 5 (PRDX5) is an atypical 2-Cys peroxiredoxin found in mitochondria, peroxisomes and the cytosol. It reduces hydrogen peroxide, alkyl hydroperoxides and peroxynitrite, helping protect cells against oxidative and nitrosative stress, and it is used as a marker in studies of redox balance and organelle protection.
| Target | Peroxiredoxin-5, mitochondrial (gene PRDX5; UniProt P30044, human) |
|---|---|
| Clone | 22P75 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 22.1 kDa |
| Observed MW | 17 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:80 |
Samples with a confirmed band (WB): human HepG2 cells, human HeLa cells, human PC-3 cells, human MCF-7 cells, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human peroxiredoxin 5.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
