| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cysteine protease ATG4B;3.4.22.-;AUT-like 1 cysteine endopeptidase;Autophagin-1;Autophagy-related cysteine endopeptidase 1;Autophagy-related protein 4 homolog B;hAPG4B;ATG4B;APG4B, AUTL1, KIAA0943; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Peroxiredoxin 5 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-PRDX5 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 22P75; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, IP, Flow (as provided in the source record). Boster Bio Anti-Peroxiredoxin 5 Rabbit Monoclonal Antibody catalog # M02891-1. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: PRDX5 (Cysteine protease ATG4B).
- Antibody format: Monoclonal; clone 22P75; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
PRDX5 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C- terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms. . Reported cellular localization context: Cytoplasm . Tissue expression notes (as provided): Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas. .
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Cardiovascular,Cell Biology,Cell Death,Extracellular Matrix,Invasion/Microenvironment,Metabolism,Metabolism Processes,Pathways and Processes,Proteolysis/Ubiquitin,Proteolytic Enzymes,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate PRDX5 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect PRDX5 expression by Western blot in cell or tissue lysates, Detect PRDX5 in FFPE tissue sections by immunohistochemistry, Quantify PRDX5-positive cells by flow cytometry in single-cell suspensions, Enrich PRDX5 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 17 kDa; calculated MW: 44294 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 17 kDa
- Cellular localization (provided): Cytoplasm .
- Tissue details (provided): Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
