| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cysteine protease ATG4C;3.4.22.-;AUT-like 3 cysteine endopeptidase;Autophagin-3;Autophagy-related cysteine endopeptidase 3;Autophagy-related protein 4 homolog C;ATG4C;APG4C, AUTL1, AUTL3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human PFDN5 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-PFDN5 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 18P17; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, IP (as provided in the source record). Boster Bio Anti-PFDN5 Rabbit Monoclonal Antibody catalog # M09908. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: PFDN5 (Cysteine protease ATG4C).
- Antibody format: Monoclonal; clone 18P17; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
PFDN5 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation (By similarity). Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms. . Reported cellular localization context: Cytoplasm . Tissue expression notes (as provided): Highly expressed in skeletal muscle, heart, liver and testis. .
Research relevance and current trends
- Research context keywords from the source record include: Metabolism Processes,Oncoproteins,Oncoproteins/Suppressors,Pathways and Processes,Proteolysis/Ubiquitin,Proteolytic Enzymes,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate PFDN5 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect PFDN5 expression by Western blot in cell or tissue lysates, Detect PFDN5 in FFPE tissue sections by immunohistochemistry, Enrich PFDN5 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 60 kDa; calculated MW: 52497 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 60 kDa
- Cellular localization (provided): Cytoplasm .
- Tissue details (provided): Highly expressed in skeletal muscle, heart, liver and testis. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
