| Field | Specification |
|---|---|
| Target | |
| Alternative names | 6-phosphogluconate dehydrogenase, decarboxylating; PGD; PGDH |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PGD recombinant protein (Position: D24-R447). Human PGD shares 93.9% and 92.9% amino acid (aa) sequence identity with mouse and rat PGD, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects 6-phosphogluconate dehydrogenase, decarboxylating (gene PGD) in human, mouse and rat samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 53.1 kDa, and the supplier reports an observed band at about 48 kDa.
6-phosphogluconate dehydrogenase (PGD) catalyzes the third step of the oxidative pentose phosphate pathway, oxidatively decarboxylating 6-phosphogluconate to yield ribulose 5-phosphate, CO2 and NADPH. It thus supplies NADPH for antioxidant defense and biosynthesis as well as pentose sugars for nucleotides, and it is studied in redox and cancer metabolism.
| Target | 6-phosphogluconate dehydrogenase, decarboxylating (gene PGD; UniProt P52209, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 53.1 kDa |
| Observed MW | 48 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells, human HepG2 cells, human A549 cells, rat liver tissue, rat PC-12 cells, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A431 cells.
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Recombinant human PGD fragment (Asp24–Arg447), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 93.9% and 92.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 6-phosphogluconate dehydrogenase, decarboxylating in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jiani Zhan et al. (2025) IDO1-mediated AhR activation up-regulates pentose phosphate pathway via NRF2 to inhibit ferroptosis in lung cancer. Biochemical Pharmacology. 10.1016/j.bcp.2025.116913
