| Field | Specification |
|---|---|
| Target | |
| Alternative names | Placenta growth factor; PlGF; PGFL; PLGF; PGF |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human PGF recombinant protein (Position: E37-D60). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes placenta growth factor (gene PGF) in human and mouse samples and is validated for ELISA and Western blot. The predicted molecular weight is 24.8 kDa, and the supplier reports an observed band at about 25 kDa.
Placental growth factor (PlGF, PGF) is a VEGF family member that binds VEGFR-1 (FLT1), and some isoforms also bind neuropilins. It supports angiogenesis and endothelial growth, particularly in pathological conditions, and is highly expressed in the placenta; circulating PlGF, often measured with sFlt-1, is widely studied in pregnancy research, including preeclampsia.
| Target | Placenta growth factor (gene PGF; UniProt P49763, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 24.8 kDa |
| Observed MW | 25 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human T-47D cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant human PGF fragment (Glu37–Asp60), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt describes placenta growth factor as a secreted protein. Tissue expression noted by UniProt (human): While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yali Xu et al. (2011) Increased expression of placental growth factor in patients with temporal lobe epilepsy and a rat model. Brain Research. 10.1016/j.brainres.2011.10.008
