| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphoglycerate kinase 1;2.7.2.3;Cell migration-inducing gene 10 protein;Primer recognition protein 2;PRP 2;PGK1;PGKA;MIG10, OK/SW-cl.110; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PGK1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EAB-16 is a rabbit monoclonal antibody that recognizes phosphoglycerate kinase 1 (gene PGK1) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 44.6 kDa, and the supplier reports an observed band at about 45 kDa.
Phosphoglycerate kinase 1 (PGK1) catalyzes the first ATP-producing step of glycolysis, transferring a phosphate from 1,3-bisphosphoglycerate to ADP to give 3-phosphoglycerate. It is ubiquitously expressed and X-linked, and in mitochondria it can also act as a protein kinase that phosphorylates PDK1 to favor glycolysis. PGK1 is studied in cancer metabolism.
| Target | Phosphoglycerate kinase 1 (gene PGK1; UniProt P00558, human) |
|---|---|
| Clone | EAB-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 44.6 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): HepG2 cells, mouse kidney.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PGK1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places phosphoglycerate kinase 1 in the cytoplasm and mitochondrion matrix. Tissue expression noted by UniProt (human): Mainly expressed in spermatogonia. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jingjing Liu et al. (2021) Terazosin Stimulates Pgk1 to Remedy Gastrointestinal Disorders. International Journal of Molecular Sciences. 10.3390/ijms23010416
