| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphoglucomutase-like protein 5; Aciculin; Phosphoglucomutase-related protein; PGM-RP; PGM5; PGMRP |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PGM5 recombinant protein (Position: Q37-H555). Human PGM5 shares 97.5% and 96.9% amino acid (aa) sequence identity with mouse and rat PGM5, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets phosphoglucomutase-like protein 5 (gene PGM5) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 62.2 kDa, and the supplier reports an observed band at about 62 kDa.
PGM5 (also called aciculin) is a phosphoglucomutase-like protein with no detectable phosphoglucomutase activity. It is found at adherens-type junctions between cells and with the matrix, especially in muscle, where it binds dystrophin and utrophin, and it is thought to help organize myofibrils and sarcomeres.
| Target | Phosphoglucomutase-like protein 5 (gene PGM5; UniProt Q15124, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 62.2 kDa |
| Observed MW | 62 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human PC-3 cells, human U2OS cells, human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): MCF-7 cells.
Immunogen
Recombinant human PGM5 fragment (Gln37–His555), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 97.5% and 96.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places phosphoglucomutase-like protein 5 in the cell junction, cytoplasm and cell membrane. Tissue expression noted by UniProt (human): Detected in smooth and cardiac muscle at high levels and in skeletal muscle at low level. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
