| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ubiquitin carboxyl-terminal hydrolase isozyme L1;UCH-L1;3.4.19.12;6.-.-.-;Neuron cytoplasmic protein 9.5;PGP 9.5;PGP9.5;Ubiquitin thioesterase L1;UCHL1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human PGP9.5, different from the related mouse and rat sequences by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes ubiquitin carboxyl-terminal hydrolase isozyme L1 (gene UCHL1) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 24.8 kDa, and the supplier reports an observed band at about 25 kDa.
UCH-L1 (PGP9.5) is a deubiquitinating enzyme that is highly abundant in neurons. It cleaves ubiquitin from small adducts to maintain a pool of free monoubiquitin and also removes ubiquitin from several proteins, including EGFR and HIF-1α. UCH-L1 is widely used as a neuronal marker and is studied in synaptic function, neurodegeneration and brain injury.
| Target | Ubiquitin carboxyl-terminal hydrolase isozyme L1 (gene UCHL1; UniProt P09936, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 24.8 kDa |
| Observed MW | 25 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human U87 cells, human SH-SY5Y cells, rat brain tissue, rat C6 cells, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue, rat brain tissue, human glioma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): SH-SY5Y cells.
Samples with a confirmed signal (flow cytometry): K562 cells.
Immunogen
Synthetic peptide from the C-terminal region of human PGP9.5. It differs from the mouse and rat sequences at two residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ubiquitin carboxyl-terminal hydrolase isozyme L1 in the cytoplasm and endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Found in neuronal cell bodies and processes throughout the neocortex (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Atherton Megan A. et al. (2022) Sympathetic modulation of tumor necrosis factor alpha-induced nociception in the presence of oral squamous cell carcinoma. Pain. 10.1097/j.pain.0000000000002655
Nguyen Huu Tu et al. (2021) Cathepsin S Evokes PAR2-Dependent Pain in Oral Squamous Cell Carcinoma Patients and Preclinical Mouse Models. Cancers. 10.3390/cancers13184697
Rui Yang et al. (2023) Expression of Phospholipase D Family Member 6 in Bovine Testes and Its Molecular Characteristics. International Journal of Molecular Sciences. 10.3390/ijms241512172
Lei Tian et al. (2019) UCH-L1 mitigates neurotoxicity induced by ZnO particles via stabilizing the inhibitor of NF-kappa B signaling, IκB-α. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2019.03.058
