Anti-PHD2/EGLN1 Antibody Picoband®

SKU:BHA21011508
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    Overview
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    EGLN1 antibody (Rabbit, polyclonal, Rabbit IgG) for Human, Mouse, Rat reactivity; supports WB, IHC, ICC, FC, IP, ELISA workflows.
    Target EGLN1
    Host Rabbit
    Reactivity Human, Mouse, Rat
    Isotype Rabbit IgG
    Application(s) WB, IHC, ICC, FC, IP, ELISA
    Available Options

    Select the variant that best fits your experiment. Availability and lead time may vary by option.

    • Options:
      • Size: 100 ug/vial; Conjugation: Unconjugated / Size: 100 ug/vial; Conjugation: Carrier Free
        • Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
        • Form: Lyophilized
        • Applications: WB,IP,Flow Cytometry
        • Application details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
          Immunoprecipitation, 0.5-2 μg/ml, Human
          Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
        • Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
      • Size: 100 ug/vial; Conjugation: Biotin / Size: 100 ug/vial; Conjugation: Cy3 / Size: 100 ug/vial; Conjugation: Fluoro488 / Size: 100 ug/vial; Conjugation: Fluoro550 / Size: 100 ug/vial; Conjugation: Fluoro594 / Size: 100 ug/vial; Conjugation: FITC / Size: 100 ug/vial; Conjugation: APC / Size: 100 ug/vial; Conjugation: PE / Size: 100 ug/vial; Conjugation: Fluoro647
        • Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
        • Form: Liquid
        • Applications: WB,IHC,ELISA
        • Application details: Western blot, 0.25-0.5μg/ml
          Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
          ELISA, 0.1-0.5μg/ml
        • Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
      • Size: 100 ug/vial; Conjugation: HRP
        • Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
        • Form: Liquid
        • Applications: WB,IHC,ELISA
        • Application details: Western blot, 0.25-0.5μg/ml
          Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
          ELISA, 0.1-0.5μg/ml
        • Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
    • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
    • Storage: Refer to the product datasheet for storage and handling.
    • Shipping: cold-chain shipment (typically with ice packs).
    • Upon receipt: follow the product datasheet storage instructions.
    • Sales terms and conditions: Please review prior to ordering.
    Options selector
    Catalog no. Size Conjugation
    A00415-1 100 ug/vial
    Field Specification
    Alternative Names EGLN1; C1orf12; PNAS-118; PNAS-137; Egl nine homolog 1; EC 1.14.11.29; Hypoxia-inducible factor prolyl hydroxylase 2; HIF-PH2; HIF-prolyl hydroxylase 2; HPH-2; Prolyl hydroxylase domain-containing protein 2; PHD2; SM-20
    Clonality
    • Polyclonal
    Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
    Gene ID 54583
    Host Rabbit
    Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human PHD2/EGLN1. Human PHD2/EGLN1 shares 100% amino acid (aa) sequence identity with both mouse and rat PHD2/EGLN1.
    Isotype
    • Rabbit IgG
    Molecular Weight 46 kDa
    Product Type
    • Antibodies
    • Primary Antibodies
    Reactivity
    • Human
    • Mouse
    • Rat
    Reconstitution Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
    Target EGLN1
    UniProt # Q9GZT9

    Boster Bio Anti-PHD2/EGLN1 Antibody Picoband® catalog # A00415-1. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Scientific background: The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).

    Cross reactivity: No cross-reactivity with other proteins

    Protein: Egl nine homolog 1 | Gene: egl-9 family hypoxia inducible factor 1 | Target: EGLN1

    Host: Rabbit | Clonality/Clone: Polyclonal | Isotype: Rabbit IgG | Reactivity: Human,Mouse,Rat | Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PHD2/EGLN1. Human PHD2/EGLN1 shares 100% amino acid (aa) sequence identity with both mouse and rat PHD2/EGLN1. | Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. | Purification: Immunogen affinity purified.

    Applications: WB, IHC, ICC, FC, IP, ELISA

    Observed MW: 46 kDa | Calculated MW: 46 kDa

    Status: Active

    Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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