| Field | Specification |
|---|---|
| Target | |
| Alternative names | PHD finger protein 11; BRCA1 C-terminus-associated protein; Renal carcinoma antigen NY-REN-34; PHF11; BCAP |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PHF11 recombinant protein (Position: M1-K284). Human PHF11 shares 57.4% and 57.3% amino acid (aa) sequence identity with mouse and rat PHF11, respectively |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects PHD finger protein 11 (gene PHF11) in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 37.6 kDa, and the supplier reports an observed band at about 34 kDa.
PHF11 is a PHD finger protein reported to promote expression of Th1-type cytokine genes such as IFN-γ in T cells. It has been linked in genetic studies to asthma and IgE levels, but its molecular mechanism is only partly characterized.
| Target | PHD finger protein 11 (gene PHF11; UniProt Q9UIL8, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 37.6 kDa |
| Observed MW | 34 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human Jurkat cells, human RT4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with a confirmed signal (flow cytometry): 293T cells, HeLa cells.
Immunogen
Recombinant human PHF11 fragment (Met1–Lys284), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 57.4% and 57.3%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places PHD finger protein 11 in the nucleus. Tissue expression noted by UniProt (human): Highly expressed in T and B-cells, as well as natural killer and mature dendritic cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
