| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform; PHK-gamma-M; Phosphorylase kinase subunit gamma-1; Serine/threonine-protein kinase PHKG1; EC 2.7.11.1, EC 2.7.11.26; PHKG1; PHKG |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PHKG1 recombinant protein (Position: E62-R353). Human PHKG1 shares 93.8% and 94.2% amino acid (aa) sequence identity with mouse and rat PHKG1, respectively. |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform (gene PHKG1) in human samples and is validated for Western blot, flow cytometry and ELISA.
PHKG1 is the catalytic gamma subunit of phosphorylase kinase in skeletal muscle and heart. Phosphorylase kinase converts glycogen phosphorylase b into the active form a in response to neural and hormonal signals such as Ca2+ and cAMP, triggering glycogen breakdown, and PHKG1 is studied in glycogen metabolism and muscle energetics.
| Target | Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform (gene PHKG1; UniProt Q16816, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human PHKG1 fragment (Glu62–Arg353), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 93.8% and 94.2%, respectively.
Reactivity Notes
The supplier lists reactivity with human.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
