| Field | Specification |
|---|---|
| Target | |
| Alternative names | RAC-alpha serine/threonine-protein kinase;2.7.11.1;Protein kinase B;PKB;Protein kinase B alpha;PKB alpha;Proto-oncogene c-Akt;RAC-PK-alpha;AKT1;PKB, RAC; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-Akt (Ser473) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IFD-1 is a rabbit monoclonal antibody that recognizes RAC-alpha serine/threonine-protein kinase (gene AKT1) phosphorylated at Ser473 in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 55.7 kDa, and the supplier reports an observed band at about 60 kDa.
AKT1 (protein kinase Bα) is one of three AKT serine/threonine kinases activated downstream of PI3K by growth factors and insulin. By phosphorylating many substrates, it promotes cell survival, growth, proliferation, glucose uptake and angiogenesis, and it is one of the most studied nodes in cancer and metabolic signaling.
| Target | RAC-alpha serine/threonine-protein kinase (gene AKT1; UniProt P31749, human) — phosphorylated at Ser473 |
|---|---|
| Clone | IFD-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 55.7 kDa |
| Observed MW | 60 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:100–1:500 |
Samples with confirmed staining (IHC): human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic phosphopeptide based on human Phospho-Akt (Ser473).
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places RAC-alpha serine/threonine-protein kinase in the cytoplasm, nucleus, cell membrane and mitochondrion intermembrane space. Tissue expression noted by UniProt (human): Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Aslıhan ŞENGELEN et al. (2024) Rosmarinic acid attenuates glioblastoma cells and spheroids’ growth and EMT/stem-like state by PTEN/PI3K/AKT downregulation and ERK-induced apoptosis. Phytomedicine. 10.1016/j.phymed.2024.156060
Evren Önay Uçar et al. (2023) Hsp27, Hsp60, Hsp70, or Hsp90 depletion enhances the antitumor effects of resveratrol via oxidative and ER stress response in human glioblastoma cells. Biochemical Pharmacology. 10.1016/j.bcp.2022.115409
Chen Guo et al. (2026) BEZ235 enhances the photodynamic antitumor efficacy of hypoxia-responsive BODIPY prodrug nanoparticles by inhibiting the PI3K/mTOR pathway and alleviating hypoxia. Colloids and Surfaces B-Biointerfaces. 10.1016/j.colsurfb.2026.115644
Qin Wu et al. (2022) Propionate ameliorates diabetes-induced neurological dysfunction through regulating the PI3K/Akt/eNOS signaling pathway. European Journal of Pharmacology. 10.1016/j.ejphar.2022.174974
Guanyu Yang et al. (2019) Fucoxanthin Alleviates Oxidative Stress through Akt/Sirt1/FoxO3α Signaling to Inhibit HG-Induced Renal Fibrosis in GMCs. Marine Drugs. 10.3390/md17120702
Shymaa I.A. Abdel-dayem et al. (2020) Sesquiterpene lactones; Damsin and neoambrosin suppress cytokine-mediated inflammation in complete Freund's adjuvant rat model via shutting Akt/ERK1/2/STAT3 signaling. Journal of Ethnopharmacology. 10.1016/j.jep.2020.113407
Gözdenur Güneş et al. (2026) Crocin Ameliorates Testicular Damage in Type 1 Diabetic Rats: Role of Akt-Mediated Nrf-2 Activation. Journal of Biochemical and Molecular Toxicology. 10.1002/jbt.70890
Yutong Yan et al. (2022) Estradiol Regulates the Expression and Secretion of Antimicrobial Peptide S100A7 via the ERK1/2-Signaling Pathway in Goat Mammary Epithelial Cells. Animals. 10.3390/ani12223077
Xie Zuolian et al. (2026) Bioinformatic and experimental analysis reveals ITGA5 as a driver of ovarian clear cell carcinoma progression and platinum resistance. 3 Biotech. 10.1007/s13205-026-04847-3
Miao Yuyang et al. (2022) Effects of 4-vinylcyclohexene diepoxide on the cell cycle, apoptosis, and steroid hormone secretion of goat ovarian granulosa cells. In Vitro Cellular & Developmental Biology-Animal. 10.1007/s11626-022-00663-0
