| Field | Specification |
|---|---|
| Target | |
| Alternative names | RAC-alpha serine/threonine-protein kinase;2.7.11.1;Protein kinase B;PKB;Protein kinase B alpha;PKB alpha;Proto-oncogene c-Akt;RAC-PK-alpha;AKT1;PKB, RAC; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human AKT1 |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HIA-1 is a rabbit monoclonal antibody that recognizes RAC-alpha serine/threonine-protein kinase (gene AKT1) phosphorylated at Ser124 in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 55.7 kDa, and the supplier reports an observed band at about 60 kDa.
AKT1 (protein kinase Bα) is one of three AKT serine/threonine kinases activated downstream of PI3K by growth factors and insulin. By phosphorylating many substrates, it promotes cell survival, growth, proliferation, glucose uptake and angiogenesis, and it is one of the most studied nodes in cancer and metabolic signaling.
| Target | RAC-alpha serine/threonine-protein kinase (gene AKT1; UniProt P31749, human) — phosphorylated at Ser124 |
|---|---|
| Clone | HIA-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 55.7 kDa |
| Observed MW | 60 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human AKT1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places RAC-alpha serine/threonine-protein kinase in the cytoplasm, nucleus, cell membrane and mitochondrion intermembrane space. Tissue expression noted by UniProt (human): Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yue Peng et al. (2026) Rational design of curcumin-borneol hybrids for rheumatoid arthritis pathological intervention via AKT/mTOR/HIF-1α pathway modulation. Materials & Design. 10.1016/j.matdes.2026.116214
Sihao Zhu et al. (2024) Black phosphorus quantum dots induce lipid accumulation through PPARγ activation and mitochondrial dysfunction in adipocytes. Science of the Total Environment. 10.1016/j.scitotenv.2024.177972
Shi Yongjing et al. (2024) The E3 ubiquitin ligase FBXO38 maintains the antitumor function of natural killer cells by sustaining IL-15R signaling. Cancer Immunology Research. 10.1158/2326-6066.CIR-23-1061
Lu Kong et al. (2021) Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells. Environmental Pollution. 10.1016/j.envpol.2021.116556
Jingjing Liu et al. (2022) Integration of pharmacodynamics and metabolomics reveals the therapeutic effects of 6-acetylacteoside on ovariectomy-induced osteoporosis mice. Phytomedicine. 10.1016/j.phymed.2022.154399
Xie Feng et al. (2021) Anti-Myocardial Ischemia Reperfusion Injury Mechanism of Dried Ginger-Aconite Decoction Based on Network Pharmacology. Frontiers in Pharmacology. 10.3389/fphar.2021.609702
Yongya Wu et al. (2020) Effect and mechanism of PI3K/AKT/mTOR signaling pathway in the apoptosis of GC-1 cells induced by nickel nanoparticles. Chemosphere. 10.1016/j.chemosphere.2020.126913
Xiao-feng Wang et al. (2025) ApoE-T807-modified tanshinone IIA liposomes enhance cognitive function in APP/PS1 mice by inhibiting Tau phosphorylation via the PI3K/AKT signaling pathway. Journal of Drug Delivery Science and Technology. 10.1016/j.jddst.2025.107219
Yue Xu et al. (2021) Mechanism of Action of Xiaoyao San in Treatment of Ischemic Stroke is Related to Anti-Apoptosis and Activation of PI3K/Akt Pathway. Drug Design Development and Therapy. 10.2147/DDDT.S280217
Jia-zhe Lin et al. (2023) A prognostic biomarker NRG1 promotes U‑87 MG glioblastoma cell malignancy by inhibiting autophagy via ERBB2/AKT/mTOR pathway. Journal of Cellular Biochemistry. 10.1002/jcb.30444
