| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alpha-synuclein;Non-A beta component of AD amyloid;Non-A4 component of amyloid precursor;NACP;SNCA;NACP, PARK1; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-alpha Synuclein (S129) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BBF-19 is a rabbit monoclonal antibody that recognizes alpha-synuclein (gene SNCA) phosphorylated at Ser129 in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 14.5 kDa, and the supplier reports an observed band at about 14 kDa.
Alpha-synuclein (SNCA) is an abundant presynaptic protein that helps regulate synaptic vesicle trafficking and neurotransmitter release, partly by promoting SNARE complex assembly. Misfolded, aggregated alpha-synuclein is the main component of Lewy bodies, so it is a central protein in Parkinson's disease and related synucleinopathy research.
| Target | Alpha-synuclein (gene SNCA; UniProt P37840, human) — phosphorylated at Ser129 |
|---|---|
| Clone | BBF-19 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 14.5 kDa |
| Observed MW | 14 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): 293T cells, 293T cells transfected with Polo-Like Kinase 2 and alpha Synuclein.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic phosphopeptide around Ser129 of human alpha Synuclein.
Reactivity Notes
The supplier lists reactivity with human. UniProt places alpha-synuclein in the cytoplasm, membrane, nucleus and synapse. Tissue expression noted by UniProt (human): Highly expressed in presynaptic terminals in the central nervous system. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Delprete Cecilia et al. (2025) Evaluation of 6 monoclonal antibodies against Ser129-phosphorylated α-synuclein: Critical role of proteinase K antigen retrieval and superior sensitivity of the D1R1R clone in human skin biopsies. Journal of Neuropathology and Experimental Neurology. 10.1093/jnen/nlaf036
