Anti-Phospho-B Raf (T401) Rabbit Monoclonal Antibody

Overview

Anti-Phospho-B Raf (T401) Rabbit Monoclonal Antibody is an antibody targeting BRAF. Common applications include WB, ICC, IF, IP. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: IBE-2; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 90-100 kDa; calculated MW: 84437 MW.

Boster Bio Anti-Phospho-B Raf (T401) Rabbit Monoclonal Antibody catalog # P00075. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: BRAF — Serine/threonine-protein kinase B-raf
• Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: IBE-2; Isotype: Rabbit IgG
• Species reactivity: Human,Mouse,Rat
• Molecular weight guidance: Observed: 90-100 kDa; Calculated: 84437 MW
• Phospho site(s): T401

Biological background

Protein function (datasheet): Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. .

Cellular localization (datasheet): Nucleus . Cytoplasm. Cell membrane . Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes. .

Tissue details (datasheet): Brain and testis.

Research relevance and current trends

• Commonly studied in contexts related to Cancer Metabolism,Cytoplasmic,Epigenetics and Nuclear Signaling,Metabolism,Metabolism Processes,Nuclear Signaling,Nuclear Signaling Pathways,Pathways and Processes,Response To Hypoxia,Signal Transduction,Signaling Pathway,Stem Cells,TGF Beta.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.