| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase B-raf;2.7.11.1;Proto-oncogene B-Raf;p94;v-Raf murine sarcoma viral oncogene homolog B1;BRAF;BRAF1, RAFB1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-B Raf (T401) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IBE-2 is a rabbit monoclonal antibody that recognizes serine/threonine-protein kinase B-raf (gene BRAF) phosphorylated at Thr401 in human, mouse and rat samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 84.4 kDa, and the supplier reports an observed band at about 84 kDa.
B-Raf (BRAF) is a RAF family serine/threonine kinase activated by RAS that phosphorylates MEK1/2 (MAP2K1/2), which in turn activate ERK in the MAPK pathway controlling cell growth and differentiation. The V600E mutation constitutively activates BRAF and is common in melanoma, thyroid and colorectal cancers, making BRAF a major drug target.
| Target | Serine/threonine-protein kinase B-raf (gene BRAF; UniProt P15056, human) — phosphorylated at Thr401 |
|---|---|
| Clone | IBE-2 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 84.4 kDa |
| Observed MW | 84 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human K562 cells, human RT4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic phosphopeptide around Thr401 of human B-Raf.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places serine/threonine-protein kinase B-raf in the nucleus, cytoplasm and cell membrane. Tissue expression noted by UniProt (human): Brain and testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
