| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bcl2-associated agonist of cell death; BAD; Bcl-2-binding component 6; Bcl-2-like protein 8; Bcl2-L-8; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death; BBC6; BCL2L8 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Bad Promotes cell death. Successfully competes for the binding to Bcl-X (L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AEBH-2 is a rabbit monoclonal antibody that recognizes bcl2-associated agonist of cell death (gene BAD) phosphorylated at Ser112 in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 18.4 kDa, and the supplier reports an observed band at about 23 kDa.
BAD is a pro-apoptotic BH3-only member of the Bcl-2 family. When dephosphorylated it binds Bcl-2 and Bcl-xL and frees BAX and BAK to trigger apoptosis, while survival signals such as AKT phosphorylate BAD and sequester it with 14-3-3 proteins. It is widely used to study growth factor-dependent cell survival.
| Target | Bcl2-associated agonist of cell death (gene BAD; UniProt Q92934, human) — phosphorylated at Ser112 |
|---|---|
| Clone | AEBH-2 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 18.4 kDa |
| Observed MW | 23 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human bad Promotes cell death.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places bcl2-associated agonist of cell death in the mitochondrion outer membrane and cytoplasm. Tissue expression noted by UniProt (human): Expressed in a wide variety of tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
