| Field | Specification |
|---|---|
| Target | |
| Alternative names | Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; p130cas; BCAR1; CAS; CASS1; CRKAS |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-BCAR1 (Y410) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 32B03 is a rabbit monoclonal antibody that recognizes breast cancer anti-estrogen resistance protein 1 (gene BCAR1) phosphorylated at Tyr410 in human samples and is validated for Western blot. The predicted molecular weight is 93.4 kDa, and the supplier reports an observed band at about 130 kDa.
BCAR1 (p130Cas) is a docking protein at focal adhesions that becomes tyrosine phosphorylated by FAK and SRC after integrin engagement. Its phosphorylated substrate domain recruits CRK and other adaptors that drive actin remodeling and cell migration. BCAR1 was identified through resistance to antiestrogens in breast cancer cells and is widely studied in adhesion signaling.
| Target | Breast cancer anti-estrogen resistance protein 1 (gene BCAR1; UniProt P56945, human) — phosphorylated at Tyr410 |
|---|---|
| Clone | 32B03 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 93.4 kDa |
| Observed MW | 130 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic phosphopeptide around Tyr410 of human BCAR1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places breast cancer anti-estrogen resistance protein 1 in the cell junction, cytoplasm and cell projection. Tissue expression noted by UniProt (human): Expressed in B-cells (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
