| Field | Specification |
|---|---|
| Target | |
| Alternative names | Catenin beta-1; Beta-catenin; CTNNB1; CTNNB |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-beta Catenin (T41 + S45) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 32C69 is a rabbit monoclonal antibody that recognizes catenin beta-1 (gene CTNNB1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 85.5 kDa, and the supplier reports an observed band at about 92 kDa.
β-Catenin (CTNNB1) has two roles: it is a structural part of cadherin-based adherens junctions and the central effector of canonical Wnt signaling. Without Wnt, a destruction complex of APC, AXIN, CK1α and GSK3B targets it for proteasomal degradation; with Wnt, it builds up in the nucleus and activates TCF/LEF target genes. It is studied in development, stem cells and cancer.
| Target | Catenin beta-1 (gene CTNNB1; UniProt P35222, human) |
|---|---|
| Clone | 32C69 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 85.5 kDa |
| Observed MW | 92 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Immunogen
Synthetic phosphopeptide around Thr41 of human beta-catenin +.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places catenin beta-1 in the cytoplasm, nucleus, cell junction and cell membrane. Tissue expression noted by UniProt (human): Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
