| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tyrosine-protein kinase BTK;2.7.10.2;Agammaglobulinemia tyrosine kinase;ATK;B-cell progenitor kinase;BPK;Bruton tyrosine kinase;BTK;AGMX1, ATK, BPK; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-BTK (Y551) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FII-2 is a rabbit monoclonal antibody that recognizes tyrosine-protein kinase BTK phosphorylated at Tyr551 in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 76.3 kDa, and the supplier reports an observed band at about 74 kDa.
Bruton′s tyrosine kinase (BTK) is a TEC family kinase that relays B-cell receptor signals to PLCγ2, calcium release and NF-κB activation, and it also acts downstream of Fc and Toll-like receptors in myeloid cells. BTK is essential for B-cell development, its mutations cause X-linked agammaglobulinemia, and it is the target of inhibitors such as ibrutinib.
| Target | Tyrosine-protein kinase BTK (gene BTK; UniProt Q06187, human) — phosphorylated at Tyr551 |
|---|---|
| Clone | FII-2 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 76.3 kDa |
| Observed MW | 74 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): Ramos cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic phosphopeptide around Tyr551 of human BTK.
Reactivity Notes
The supplier lists reactivity with human. UniProt places tyrosine-protein kinase BTK in the cytoplasm, cell membrane, nucleus and membrane raft. Tissue expression noted by UniProt (human): Predominantly expressed in B-lymphocytes. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
