| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transcription factor AP-1;Activator protein 1;AP1;Proto-oncogene c-Jun;V-jun avian sarcoma virus 17 oncogene homolog;p39;JUN; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-c-Jun (S73)+JunD (S100) |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 30J61 is a rabbit monoclonal antibody that recognizes transcription factor Jun phosphorylated at Ser73 in human and mouse samples and is validated for Western blot, IHC and immunoprecipitation.
c-Jun (JUN) is a bZIP transcription factor and core component of AP-1. It forms dimers with Fos family proteins and other partners to bind TRE (TGA[GC]TCA) sites and control genes involved in proliferation, differentiation, stress responses and apoptosis. Its activity is strongly increased by JNK phosphorylation, and it is a widely used readout in studies of cell signaling and cancer.
| Target | Transcription factor Jun (gene JUN; UniProt P05412, human) — phosphorylated at Ser73 |
|---|---|
| Clone | 30J61 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Observed MW | 38, 42, 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): RAW 2647 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic phosphopeptide around Ser73 of human c-Jun+JunD.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places transcription factor Jun in the nucleus. Tissue expression noted by UniProt (human): Expressed in the developing and adult prostate and prostate cancer cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
