| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hsp90 co-chaperone Cdc37;Hsp90 chaperone protein kinase-targeting subunit;p50Cdc37;Hsp90 co-chaperone Cdc37, N-terminally processed;CDC37;CDC37A; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-CDC37 (S13) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HIF-3 is a rabbit monoclonal antibody that recognizes hsp90 co-chaperone Cdc37 phosphorylated at Ser13 in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 44.5 kDa, and the supplier reports an observed band at about 50 kDa.
CDC37 is an Hsp90 co-chaperone that recognizes many protein kinases and delivers them to Hsp90 for folding and stabilization. Because a large share of cellular kinases depend on this system, CDC37 is studied in kinase signaling, protein homeostasis and cancer cell survival.
| Target | Hsp90 co-chaperone Cdc37 (gene CDC37; UniProt Q16543, human) — phosphorylated at Ser13 |
|---|---|
| Clone | HIF-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 44.5 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:20 |
Immunogen
Synthetic phosphopeptide around Ser13 of human CDC37.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places hsp90 co-chaperone Cdc37 in the cytoplasm. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
