| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | G1/S-specific cyclin-E1;CCNE1;CCNE; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human Cyclin E1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Target | |
| UniProt # |
Overview
Anti-Phospho-Cyclin E1 (T77) CCNE1 Rabbit Monoclonal Antibody is an antibody targeting CCNE1. Common applications include WB, IHC, ICC, IF. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: GHC-3; isotype: Rabbit IgG; reactivity: Human; observed MW: 185 kDa; calculated MW: 47077 MW.
Boster Bio Anti-Phospho-Cyclin E1 (T77) CCNE1 Rabbit Monoclonal Antibody catalog # P00543. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CCNE1 — G1/S-specific cyclin-E1
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: GHC-3; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 185 kDa; Calculated: 47077 MW
- Phospho site(s): T77
Biological background
Protein function (datasheet): Essential for the control of the cell cycle at the G1/S (start) transition. .
Cellular localization (datasheet): Nucleus .
Tissue details (datasheet): Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. .
Research relevance and current trends
- Commonly studied in contexts related to Apoptosis,Cancer,Cell Biology,Cell Death,Metabolism,Metabolism Processes,Mitochondrial,Mitochondrial Markers,Mitochondrial Metabolism,Oncoproteins,Oncoproteins/Suppressors,Pathways and Processes,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
