| Field | Specification |
|---|---|
| Target | |
| Alternative names | Eukaryotic translation initiation factor 2 subunit 1;Eukaryotic translation initiation factor 2 subunit alpha;eIF-2-alpha;eIF-2A;eIF-2alpha;EIF2S1;EIF2A; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-EIF2S1 (S51) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IO-5 is a rabbit monoclonal antibody that recognizes eukaryotic translation initiation factor 2 subunit 1 (gene EIF2S1) phosphorylated at Ser51 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 36.1 kDa, and the supplier reports an observed band at about 36 kDa.
eIF2α (EIF2S1) is the regulatory subunit of eIF2, the factor that brings initiator methionyl-tRNA to the ribosome. Phosphorylation of eIF2α by stress kinases such as PERK, PKR, GCN2 and HRI blocks eIF2 recycling and reduces global protein synthesis, making phospho-eIF2α a standard marker of the integrated stress response.
| Target | Eukaryotic translation initiation factor 2 subunit 1 (gene EIF2S1; UniProt P05198, human) — phosphorylated at Ser51 |
|---|---|
| Clone | IO-5 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 36.1 kDa |
| Observed MW | 36 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:40 |
Samples with a confirmed band (WB): human K562 cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic phosphopeptide around Ser51 of human EIF2S1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places eukaryotic translation initiation factor 2 subunit 1 in the cytoplasm and mitochondrion. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hongxing Li et al. (2020) MicroRNA-375 exacerbates knee osteoarthritis through repressing chondrocyte autophagy by targeting ATG2B. Aging-US. 10.18632/aging.103073
Chen Zhao et al. (2018) Blockage of High-Affinity Choline Transporter Increases Visceral Hypersensitivity in Rats with Chronic Stress. Gastroenterology Research and Practice. 10.1155/2018/9252984
