| Field | Specification |
|---|---|
| Target | |
| Alternative names | Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I; EIF4EBP1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-eIF4EBP1 (T70) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 32E48 is a rabbit monoclonal antibody that recognizes eukaryotic translation initiation factor 4E-binding protein 1 (gene EIF4EBP1) phosphorylated at Thr70 in human samples and is validated for Western blot. The predicted molecular weight is 12.6 kDa, and the supplier reports an observed band at about 17 kDa.
4E-BP1 (EIF4EBP1) is a translational repressor that binds eIF4E and prevents it from joining eIF4G. When mTORC1 phosphorylates 4E-BP1, it releases eIF4E and cap-dependent translation increases, so phospho-4E-BP1 is a standard readout of mTOR activity.
| Target | Eukaryotic translation initiation factor 4E-binding protein 1 (gene EIF4EBP1; UniProt Q13541, human) — phosphorylated at Thr70 |
|---|---|
| Clone | 32E48 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 12.6 kDa |
| Observed MW | 17 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human SH-SY5Y cells, human HEL cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic phosphopeptide around Thr70 of human eIF4EBP1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places eukaryotic translation initiation factor 4E-binding protein 1 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
YiQiang Li et al. (2017) mTORC1 signaling is essential for neurofibromatosis type I gene modulated osteogenic differentiation of BMSCs. Journal of Cellular Biochemistry. 10.1002/jcb.26626
