| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitogen-activated protein kinase 3;MAP kinase 3;MAPK 3;2.7.11.24;ERT2;Extracellular signal-regulated kinase 1;ERK-1;Insulin-stimulated MAP2 kinase;MAP kinase isoform p44;p44-MAPK;Microtubule-associated protein 2 kinase;p44-ERK1;MAPK3;ERK1, PRKM3; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IH-13 is a rabbit monoclonal antibody that recognizes mitogen-activated protein kinase 3 (gene MAPK3) phosphorylated at Thr202/Tyr204 in human samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 43.1 kDa; the supplier reports observed bands at 43, 41 kDa.
ERK1 (MAPK3) is a serine/threonine kinase closely related to ERK2 at the end of the RAS–RAF–MEK–ERK cascade. Once activated by MEK1/2, it phosphorylates many cytoplasmic and nuclear targets that control proliferation, differentiation and survival, and dual phosphorylation of ERK1/2 is a standard marker of pathway activation.
| Target | Mitogen-activated protein kinase 3 (gene MAPK3; UniProt P27361, human) — phosphorylated at Thr202/Tyr204 |
|---|---|
| Clone | IH-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 43.1 kDa |
| Observed MW | 43, 41 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:40 |
Samples with a confirmed band (WB): A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic phosphopeptide around Thr202 of human erk1 + Erk2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places mitogen-activated protein kinase 3 in the cytoplasm, nucleus, membrane and cell junction. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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