| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glycogen synthase kinase-3 alpha;GSK-3 alpha;2.7.11.26;Serine/threonine-protein kinase GSK3A;2.7.11.1;GSK3A; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-GSK3 (alpha + beta) (Y216 + Y279) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IFB-7 is a rabbit monoclonal antibody that recognizes glycogen synthase kinase-3 alpha (gene GSK3A) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 51.0 kDa; the supplier reports observed bands at 47,51 kDa.
Glycogen synthase kinase-3 alpha (GSK3A) is a constitutively active serine/threonine kinase that is switched off by upstream signals such as insulin-activated AKT. It phosphorylates and inhibits glycogen synthase and targets β-catenin and other substrates in the Wnt pathway. Together with its paralog GSK3B, it is studied in glucose metabolism, Wnt signaling and neuronal function.
| Target | Glycogen synthase kinase-3 alpha (gene GSK3A; UniProt P49840, human) |
|---|---|
| Clone | IFB-7 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 51.0 kDa |
| Observed MW | 47,51 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human A549 cells, human RT4 cells, human 293T cells, human SH-SY5Y cells, human U2OS cells, human SIHA cells, rat thymus tissue, rat brain tissue, rat heart tissue, rat PC-12 cells, mouse thymus tissue, mouse brain tissue, mouse heart tissue, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic phosphopeptide around Tyr216 of human GSK3 (alpha + beta).
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liang Yao et al. (2026) KCTD15 Enhances Stem Cell‐Like Properties and Promotes Triple‐Negative Breast Cancer Progression Through KLF4/β‐Catenin Signaling. FASEB Journal. 10.1096/fj.202503381R
