| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Glycogen synthase kinase-3 alpha;GSK-3 alpha;2.7.11.26;Serine/threonine-protein kinase GSK3A;2.7.11.1;GSK3A; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-GSK3 (alpha + beta) (Y216 + Y279) |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Target | |
| UniProt # |
Overview
Anti-Phospho-GSK3 (alpha + beta) (Y216 + Y279) GSK3A Rabbit Monoclonal Antibody is an antibody targeting GSK3A. Common applications include WB, IHC, ICC, IF, IP. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: IFB-7; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 47, 51 kDa; calculated MW: 50981 MW.
Boster Bio Anti-Phospho-GSK3 (alpha + beta) (Y216 + Y279) GSK3A Rabbit Monoclonal Antibody catalog # P03152-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: GSK3A — Glycogen synthase kinase-3 alpha
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: IFB-7; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 47, 51 kDa; Calculated: 50981 MW
- Phospho site(s): Y216,Y279
Biological background
Protein function (datasheet): Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation. .
Cellular localization (datasheet): Cytoplasm .
Tissue details (datasheet): Restricted to the nervous system. Highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus. .
Research relevance and current trends
- Commonly studied in contexts related to Apoptosis,Cancer,Cell Biology,G Protein Signaling,Host-Virus Interaction,Interspecies Interaction,Intracellular,Kinases,Microbiology,Neural Signal Transduction,Neurology Process,Neuroscience,Protein Phosphorylation,Ras Family,Ser/Thr Kinases,Serine/Threonine Kinases,Signal Transduction,Signaling Pathway,Small G Proteins.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
