| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glycogen synthase kinase-3 beta;GSK-3 beta;2.7.11.26;Serine/threonine-protein kinase GSK3B;2.7.11.1;GSK3B; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-GSK3 beta (Ser9) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IFC-7 is a rabbit monoclonal antibody that recognizes glycogen synthase kinase-3 beta (gene GSK3B) phosphorylated at Ser9 in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 46.7 kDa, and the supplier reports an observed band at about 46 kDa.
Glycogen synthase kinase-3 beta (GSK3B) is a widely expressed, constitutively active serine/threonine kinase that is inhibited by insulin, growth factor and Wnt signals. Its substrates include glycogen synthase, β-catenin, tau and several transcription factors, placing it at the crossroads of metabolism, Wnt signaling and cytoskeletal regulation. GSK3B is heavily studied in diabetes, neurodegeneration, mood disorders and cancer.
| Target | Glycogen synthase kinase-3 beta (gene GSK3B; UniProt P49841, human) — phosphorylated at Ser9 |
|---|---|
| Clone | IFC-7 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 46.7 kDa |
| Observed MW | 46 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with confirmed staining (IHC): human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic phosphopeptide based on human Phospho-GSK3 beta (Ser9).
Reactivity Notes
The supplier lists reactivity with human. UniProt places glycogen synthase kinase-3 beta in the cytoplasm, nucleus and cell membrane. Tissue expression noted by UniProt (human): Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wenxia Li et al. (2025) Di-(2-ethylhexyl) Phthalate (DEHP) and Its Metabolite Promote Multiple Myeloma Progression and Bortezomib Resistance. Journal of Hazardous Materials. 10.1016/j.jhazmat.2025.139147
Yi Chen et al. (2026) IER3 Promotes Non-Small Cell Lung Cancer Malignancy by Suppressing Ferroptosis via the AKT/GSK3β/NRF2 Pathway. Free Radical Biology and Medicine. 10.1016/j.freeradbiomed.2026.01.029
Hangjie Fu et al. (2023) Water extract of cacumen platycladi promotes hair growth through the Akt/GSK3β/β-catenin signaling pathway. Frontiers in Pharmacology. 10.3389/fphar.2023.1038039
Ruitao Yu et al. (2025) Arctigenin from Saussurea medusa Maxim. Targets the PI3K/AKT Pathway to Inhibit Hepatocellular Carcinoma Proliferation and Induces Apoptosis. Nutrients. 10.3390/nu17193151
Sihan Feng et al. (2025) Phytochemical Composition and Skin-Friendly Activities of the Ethyl Acetate Fraction in Ophioglossum vulgatum Linn., an In Vitro Study. Pharmaceuticals. 10.3390/ph18030345
Yuan Zhou et al. (2018) Essential roles of Akt/Snail pathway in microcystin-LR-induced tight junction toxicity in Sertoli cell. Food and Chemical Toxicology. 10.1016/j.fct.2018.01.004
Ruizan Shi et al. (2020) Downregulation of cytokeratin 18 induces cellular partial EMT and stemness through increasing EpCAM expression in breast cancer. Cellular Signalling. 10.1016/j.cellsig.2020.109810
Rui Li et al. (2026) Alix Mediated Selective Packaging of β-Catenin into Extracellular Vesicles Enhances Their Pro-Angiogenic Function. Journal of Biological Chemistry. 10.1016/j.jbc.2026.111254
Miao-Yun Long et al. (2020) Comprehensive circular RNA profiling reveals the regulatory role of circRNA_0007694 in papillary thyroid carcinoma. American Journal of Translational Research. PMID: 32355548
Kuangyu Jin et al. (2026) STEAP1 Suppresses Oral Squamous Cell Carcinoma by Targeting Wnt/β‐Catenin Signalling and EMT. Cancer Medicine. 10.1002/cam4.71862
