| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heat shock factor protein 1;HSF 1;Heat shock transcription factor 1;HSTF 1;HSF1;HSTF1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-HSF1 (S326) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BHI-8 is a rabbit monoclonal antibody that recognizes heat shock factor protein 1 (gene HSF1) phosphorylated at Ser326 in human samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 57.3 kDa, and the supplier reports an observed band at about 80 kDa.
Heat shock factor 1 (HSF1) is the master transcription factor of the heat shock response. Under proteotoxic stress it forms trimers, binds heat shock elements and switches on genes for chaperones such as HSP70 and HSP90, which protect cells from misfolded proteins. HSF1 is studied in protein homeostasis, aging, neurodegeneration and cancer.
| Target | Heat shock factor protein 1 (gene HSF1; UniProt Q00613, human) — phosphorylated at Ser326 |
|---|---|
| Clone | BHI-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 57.3 kDa |
| Observed MW | 80 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human K562 cells, human HepG2 cells, human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:1000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human spleen tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic phosphopeptide around Ser326 of human HSF1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places heat shock factor protein 1 in the nucleus, cytoplasm and chromosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Li Li et al. (2023) Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination. Developmental Cell. 10.1016/j.devcel.2023.09.002
Yifan Peng-Winkler et al. (2022) Zinc supplementation prior to heat shock enhances HSP70 synthesis through HSF1 phosphorylation at serine 326 in human peripheral mononuclear cells. Food & Function. 10.1039/D2FO01406H
Xiaowen Wang et al. (2013) Molecular mechanism of polypeptides from Chlamys farreri (PCF)’s anti-apoptotic effect in UVA-exposed HaCaT cells involves HSF1/HSP70, JNK, XO, iNOS and NO/ROS. Journal of Photochemistry and Photobiology B-Biology. 10.1016/j.jphotobiol.2013.11.005
