| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Heat shock factor protein 1;HSF 1;Heat shock transcription factor 1;HSTF 1;HSF1;HSTF1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-HSF1 (S326) |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Target | |
| UniProt # |
Overview
Anti-Phospho-HSF1 (S326) Rabbit Monoclonal Antibody is an antibody targeting HSF1. Common applications include WB, IHC, ICC, IF, IP, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: BHI-8; isotype: Rabbit IgG; reactivity: Human; observed MW: 80 kDa; calculated MW: 57260 MW.
Boster Bio Anti-Phospho-HSF1 (S326) Rabbit Monoclonal Antibody catalog # P00250. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: HSF1 — Heat shock factor protein 1
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: BHI-8; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 80 kDa; Calculated: 57260 MW
- Phospho site(s): S326
Biological background
Protein function (datasheet): DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. .
Cellular localization (datasheet): Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
Tissue details (datasheet): Predominantly expressed in B-lymphocytes.
Research relevance and current trends
- Commonly studied in contexts related to Cardiogenesis,Cardiovascular,Epigenetics and Nuclear Signaling,Nucleus,Subcellular Markers,Tags & Cell Markers,Transcription,Transcription Factors/Regulators.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
