| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hormone-sensitive lipase;HSL;3.1.1.79;LIPE; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-HSL (S853) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FDO-12 is a rabbit monoclonal antibody that recognizes hormone-sensitive lipase (gene LIPE) phosphorylated at Ser853 in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 116.6 kDa, and the supplier reports an observed band at about 83 kDa.
Hormone-sensitive lipase (LIPE, HSL) is an intracellular lipase that hydrolyzes diacylglycerols, triglycerides and cholesteryl esters. In adipocytes it is activated by PKA after β-adrenergic stimulation and works after ATGL to release fatty acids during lipolysis. HSL is also important in steroidogenic tissues and is studied in fat metabolism, obesity and insulin resistance.
| Target | Hormone-sensitive lipase (gene LIPE; UniProt Q05469, human) — phosphorylated at Ser853 |
|---|---|
| Clone | FDO-12 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 116.6 kDa |
| Observed MW | 83 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): rat skeletal muscle tissue, mouse skeletal muscle tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic phosphopeptide around Ser853 of human HSL.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places hormone-sensitive lipase in the cell membrane, membrane and cytoplasm. Tissue expression noted by UniProt (human): Testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wang Xiaohui et al. (2026) Decreasing S1PR3 Alleviated Renal Tubular Lipid Deposition in Diabetic Nephropathy via AMPK/PPAR-γ/GPAT3 Pathway. Applied Biochemistry and Biotechnology. 10.1007/s12010-026-05640-x
Li Lin et al. (2024) The role of GPR81–cAMP–PKA pathway in endurance training-induced intramuscular triglyceride accumulation and mitochondrial content changes in rats. Journal of Physiological Sciences. 10.1186/s12576-024-00902-x
