| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heat shock protein beta-1;HspB1;28 kDa heat shock protein;Estrogen-regulated 24 kDa protein;Heat shock 27 kDa protein;HSP 27;Stress-responsive protein 27;SRP27;HSPB1;HSP27, HSP28; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-Hsp27 (S82) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone FGD-8 is a rabbit monoclonal antibody that recognizes heat shock protein beta-1 (gene HSPB1) phosphorylated at Ser82 in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 22.8 kDa, and the supplier reports an observed band at about 27 kDa.
HSP27 (HSPB1) is a small heat shock protein that forms large oligomers and binds unfolding proteins to prevent their aggregation. Its phosphorylation by p38 MAPK-activated kinases regulates its assembly and roles in actin organization and resistance to apoptosis, and it is a common marker of cellular stress. HSPB1 mutations cause inherited peripheral neuropathy.
| Target | Heat shock protein beta-1 (gene HSPB1; UniProt P04792, human) — phosphorylated at Ser82 |
|---|---|
| Clone | FGD-8 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 22.8 kDa |
| Observed MW | 27 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human MCF-7 cells, human HeLa cells, rat PC-12 cells, rat C6 cells, mouse HEPA1–6 cells, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic phosphopeptide around Ser82 of human hsp27.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places heat shock protein beta-1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xuyu He et al. (2016) Atorvastatin attenuates contrast-induced nephropathy by modulating inflammatory responses through the regulation of JNK/p38/Hsp27 expression. Journal of Pharmacological Sciences. 10.1016/j.jphs.2016.03.006
