| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interferon regulatory factor 3;IRF-3;IRF3; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-IRF3 (S386) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IGO-9 is a rabbit monoclonal antibody that recognizes interferon regulatory factor 3 (gene IRF3) phosphorylated at Ser386 in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 47.2 kDa, and the supplier reports an observed band at about 55 kDa.
Interferon regulatory factor 3 (IRF3) is a transcription factor that drives type I interferon production during viral infection. Sensing pathways such as RIG-I/MAVS, cGAS–STING and TLR3/4–TRIF activate the kinase TBK1, which phosphorylates IRF3 so it can dimerize, enter the nucleus and induce IFN-β and interferon-stimulated genes.
| Target | Interferon regulatory factor 3 (gene IRF3; UniProt Q14653, human) — phosphorylated at Ser386 |
|---|---|
| Clone | IGO-9 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 47.2 kDa |
| Observed MW | 55 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human SIHA cells, human RT4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic phosphopeptide around Ser386 of human IRF3.
Reactivity Notes
The supplier lists reactivity with human. UniProt places interferon regulatory factor 3 in the cytoplasm, nucleus and mitochondrion. Tissue expression noted by UniProt (human): Expressed constitutively in a variety of tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ming-Wei Fan et al. (2026) Electroacupuncture protects gastric Cajal cells by reducing macrophage pyroptosis in diabetic gastroparesis. World Journal of Diabetes. 10.4239/wjd.v17.i2.114252
Xiang Cai et al. (2024) JMJD2A promotes the development of castration-resistant prostate cancer by activating androgen receptor enhancer and inhibiting the cGAS-STING pathway. Molecular Carcinogenesis. 10.1002/mc.23753
