| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tyrosine-protein kinase Lyn;2.7.10.2;Lck/Yes-related novel protein tyrosine kinase;V-yes-1 Yamaguchi sarcoma viral related oncogene homolog;p53Lyn;p56Lyn;LYN;JTK8; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-Lyn (Y396) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IFI-12 is a rabbit monoclonal antibody that recognizes tyrosine-protein kinase Lyn phosphorylated at Tyr396 in human and mouse samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 58.6 kDa, and the supplier reports an observed band at about 56 kDa.
Lyn is a Src-family non-receptor tyrosine kinase expressed mainly in B cells and myeloid cells. It phosphorylates signaling motifs of the B-cell receptor, Fc receptors and other immune receptors, both starting and shutting down their signals; because loss of Lyn in mice leads to autoimmunity, it is studied in B-cell tolerance, allergy and hematopoietic cancers.
| Target | Tyrosine-protein kinase Lyn (gene LYN; UniProt P07948, human) — phosphorylated at Tyr396 |
|---|---|
| Clone | IFI-12 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 58.6 kDa |
| Observed MW | 56 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic phosphopeptide around Tyr396 of human lyn.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places tyrosine-protein kinase Lyn in the cell membrane, nucleus, cytoplasm and Golgi apparatus. Tissue expression noted by UniProt (human): Detected in monocytes (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Huili Zhou et al. (2024) CD11b deficiency attenuates the ischemia/reperfusion-induced AKI-to-CKD process by regulating macrophage polarization. FASEB Journal. 10.1096/fj.202402318R
