| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Dual specificity mitogen-activated protein kinase kinase 1;MAP kinase kinase 1;MAPKK 1;MKK1;2.7.12.2;ERK activator kinase 1;MAPK/ERK kinase 1;MEK 1;MAP2K1;MEK1, PRKMK1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-MCM2 (S108) |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Target | |
| UniProt # |
Boster Bio Anti-Phospho-MCM2 (S108) Rabbit Monoclonal Antibody catalog # P00374-2. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Gene: Dual specificity mitogen-activated protein kinase kinase 1 | Target: MCM2 | Function: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator- activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. . | Research areas: MAPK Pathway,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction
Host: Rabbit | Clonality/Clone: Monoclonal / Clone: 28M76 | Isotype: IgG | Reactivity: Human,Mouse,Rat | Immunogen: A synthesized peptide derived from human Phospho-MCM2 (S108) | Concentration: 0.5mg/ml | Purification: Affinity-chromatography
Applications: WB, IF, ICC, IP
Observed MW: 125 kDa | Calculated MW: 43439 MW
Cellular localization: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm. Nucleus. Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis.
Tissue details: Widely expressed, with extremely low levels in brain. .
Phospho site(s): S108
Status: Active
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