| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Retinoblastoma-associated protein;p105-Rb;pRb;Rb;pp110;RB1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-MDM2 (S166) |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Target | |
| UniProt # |
Boster Bio Anti-Phospho-MDM2 (S166) Rabbit Monoclonal Antibody catalog # P00054-2. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Gene: Retinoblastoma-associated protein | Target: MDM2 | Function: Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. ly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. . | Research areas: Cancer,Cancer Susceptibility,Cell Biology,Cell Cycle,Cell Cycle Inhibitors,Epigenetics and Nuclear Signaling,Nucleus,Oncoproteins/Suppressors,Subcellular Markers,Tags & Cell Markers,Transcription,Tumor Suppressors
Host: Rabbit | Clonality/Clone: Monoclonal / Clone: 29M35 | Isotype: IgG | Reactivity: Human,Mouse,Rat | Immunogen: A synthesized peptide derived from human Phospho-MDM2 (S166) | Concentration: 0.5mg/ml | Purification: Affinity-chromatography
Applications: WB, IHC
Observed MW: 90-100 kDa | Calculated MW: 106159 MW
Cellular localization: Nucleus .
Tissue details: Expressed in the retina.
Phospho site(s): S166
Status: Active
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