| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dual specificity mitogen-activated protein kinase kinase 1;MAP kinase kinase 1;MAPKK 1;MKK1;2.7.12.2;ERK activator kinase 1;MAPK/ERK kinase 1;MEK 1;MAP2K1;MEK1, PRKMK1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MEK1 |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HBO-13 is a rabbit monoclonal antibody that recognizes dual specificity mitogen-activated protein kinase kinase 1 (gene MAP2K1) phosphorylated at Ser298 in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 43.4 kDa, and the supplier reports an observed band at about 54 kDa.
MEK1 (MAP2K1) is a dual-specificity kinase in the RAS–RAF–MEK–ERK pathway. Activated by RAF, it phosphorylates ERK1 and ERK2 on both a threonine and a tyrosine in their activation loop, turning on the main mitogenic MAPK cascade, and it is a major target in cancer research and of clinically used MEK inhibitors.
| Target | Dual specificity mitogen-activated protein kinase kinase 1 (gene MAP2K1; UniProt Q02750, human) — phosphorylated at Ser298 |
|---|---|
| Clone | HBO-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 43.4 kDa |
| Observed MW | 54 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human HeLa cells, human A431 cells, human U251 cells, human SIHA cells, rat lung tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human MEK1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places dual specificity mitogen-activated protein kinase kinase 1 in the cytoplasm, nucleus and membrane. Tissue expression noted by UniProt (human): Widely expressed, with extremely low levels in brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Lan Ma et al. (2023) Fluoride regulates the differentiation and atrophy through FGF21/ERK signaling pathway in C2C12 cells. Ecotoxicology and Environmental Safety. 10.1016/j.ecoenv.2023.114626
Xiaoyu Chen et al. (2025) Cinnamaldehyde-surfactin nano-capsulation mitigates high-fat diet/ streptozotocin-induced diabetes and modulate gut microbial dysbiosis. Food Bioscience. 10.1016/j.fbio.2025.106735
CHENG CHENG et al. (2025) Tumor-expressing PD-L1 regulates NT5E expression through MAPK/ERK pathway in triple-negative breast cancer. Oncology Research. 10.32604/or.2025.061637
Zhou Fengfeng et al. (2026) Licochalcone a Disrupted Mitochondrial Function to Promote Cuproptosis in Glioblastoma Through Regulating RAS/MAPK Pathway. Applied Biochemistry and Biotechnology. 10.1007/s12010-026-05726-6
Wei HuaJuan et al. (2025) miR- 34c- 5p targets ROCK1 expression to inhibit kidney injury in diabetic nephropathy rats through MAPK/ERK signaling pathway. In Vitro Cellular & Developmental Biology-Animal. 10.1007/s11626-025-01039-w
