| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dual specificity mitogen-activated protein kinase kinase 1;MAP kinase kinase 1;MAPKK 1;MKK1;2.7.12.2;ERK activator kinase 1;MAPK/ERK kinase 1;MEK 1;MAP2K1;MEK1, PRKMK1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human MEK1 |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HCG-13 is a rabbit monoclonal antibody that recognizes dual specificity mitogen-activated protein kinase kinase 1 (gene MAP2K1) phosphorylated at Thr292 in human samples and is validated for Western blot. The predicted molecular weight is 43.4 kDa, and the supplier reports an observed band at about 45 kDa.
MEK1 (MAP2K1) is a dual-specificity kinase in the RAS–RAF–MEK–ERK pathway. Activated by RAF, it phosphorylates ERK1 and ERK2 on both a threonine and a tyrosine in their activation loop, turning on the main mitogenic MAPK cascade, and it is a major target in cancer research and of clinically used MEK inhibitors.
| Target | Dual specificity mitogen-activated protein kinase kinase 1 (gene MAP2K1; UniProt Q02750, human) — phosphorylated at Thr292 |
|---|---|
| Clone | HCG-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 43.4 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human MEK1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places dual specificity mitogen-activated protein kinase kinase 1 in the cytoplasm, nucleus and membrane. Tissue expression noted by UniProt (human): Widely expressed, with extremely low levels in brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
