| Field | Specification |
|---|---|
| Target | |
| Alternative names | Hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met; MET |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human c-Met |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22M56 is a rabbit monoclonal antibody that recognizes hepatocyte growth factor receptor (gene MET) phosphorylated at Tyr1349 in human samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 155.5 kDa, and the supplier reports an observed band at about 156 kDa.
MET is the receptor tyrosine kinase for hepatocyte growth factor (HGF). HGF binding activates MET and downstream pathways such as RAS-MAPK and PI3K-AKT, promoting epithelial cell scattering, migration, proliferation and survival during development and tissue repair. MET amplification, mutation or overexpression occurs in many cancers, making it a major target in cancer research.
| Target | Hepatocyte growth factor receptor (gene MET; UniProt P08581, human) — phosphorylated at Tyr1349 |
|---|---|
| Clone | 22M56 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 155.5 kDa |
| Observed MW | 156 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human PC-3 cells, human A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human c-Met.
Reactivity Notes
The supplier lists reactivity with human. UniProt places hepatocyte growth factor receptor in the membrane and SUBCELLULAR LOCATION:. Tissue expression noted by UniProt (human): Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
