| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase mTOR;2.7.11.1;FK506-binding protein 12-rapamycin complex-associated protein 1;FKBP12-rapamycin complex-associated protein;Mammalian target of rapamycin;mTOR;Mechanistic target of rapamycin;Rapamycin and FKBP12 target 1;Rapamycin target protein 1;MTOR;FRAP, FRAP1, FRAP2, RAFT1, RAPT1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-mTOR (S2448) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IFF-13 is a rabbit monoclonal antibody that recognizes serine/threonine-protein kinase mTOR phosphorylated at Ser2448 in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 288.9 kDa, and the supplier reports an observed band at about 289 kDa.
mTOR is a serine/threonine kinase that forms two complexes, mTORC1 and mTORC2, and serves as a central integrator of nutrient, growth factor and energy signals. mTORC1 drives protein, lipid and nucleotide synthesis through targets such as S6K and 4E-BP1 and suppresses autophagy. mTOR signaling is studied across cell growth, metabolism, aging, immunity and cancer.
| Target | Serine/threonine-protein kinase mTOR (gene MTOR; UniProt P42345, human) — phosphorylated at Ser2448 |
|---|---|
| Clone | IFF-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 288.9 kDa |
| Observed MW | 289 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): HEK293 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic phosphopeptide around Ser2448 of human mTOR.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places serine/threonine-protein kinase mTOR in the lysosome membrane, endoplasmic reticulum membrane and Golgi apparatus membrane. Tissue expression noted by UniProt (human): Expressed in numerous tissues, with highest levels in testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yue Peng et al. (2026) Rational design of curcumin-borneol hybrids for rheumatoid arthritis pathological intervention via AKT/mTOR/HIF-1α pathway modulation. Materials & Design. 10.1016/j.matdes.2026.116214
Lu Kong et al. (2021) Mechanisms underlying reproductive toxicity induced by nickel nanoparticles identified by comprehensive gene expression analysis in GC-1 spg cells. Environmental Pollution. 10.1016/j.envpol.2021.116556
Li Xiaoping et al. (2023) Connexin 43-modified bone marrow stromal cells reverse the imatinib resistance of K562 cells via Ca2+-dependent gap junction intercellular communication. Chinese Medical Journal. 10.1097/CM9.0000000000002554
Yang Jing-jing et al. (2026) Silencing Uracil-DNA glycosylase inhibits colorectal cancer progression. Cancer Cell International. 10.1186/s12935-025-04089-y
Yongya Wu et al. (2020) Effect and mechanism of PI3K/AKT/mTOR signaling pathway in the apoptosis of GC-1 cells induced by nickel nanoparticles. Chemosphere. 10.1016/j.chemosphere.2020.126913
Meihua Shan et al. (2017) Autophagy suppresses isoprenaline-induced M2 macrophage polarization via the ROS/ERK and mTOR signaling pathway. Free Radical Biology and Medicine. 10.1016/j.freeradbiomed.2017.05.021
Chen Guo et al. (2026) BEZ235 enhances the photodynamic antitumor efficacy of hypoxia-responsive BODIPY prodrug nanoparticles by inhibiting the PI3K/mTOR pathway and alleviating hypoxia. Colloids and Surfaces B-Biointerfaces. 10.1016/j.colsurfb.2026.115644
Xiaorui Liu et al. (2020) miR-34a/c induce caprine endometrial epithelial cell apoptosis by regulating circ-8073/CEP55 via the RAS/RAF/MEK/ERK and PI3K/AKT/mTOR pathways. Journal of Cellular Physiology. 10.1002/jcp.29821
Li Ma et al. (2022) Effect of MiR-100-5p on proliferation and apoptosis of goat endometrial stromal cell in vitro and embryo implantation in vivo. Journal of Cellular and Molecular Medicine. 10.1111/jcmm.17226
Zhang Yue et al. (2020) A Regulatory Circuit Orchestrated by Novel-miR-3880 Modulates Mammary Gland Development. Frontiers in Cell and Developmental Biology. 10.3389/fcell.2020.00383
