| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase mTOR;2.7.11.1;FK506-binding protein 12-rapamycin complex-associated protein 1;FKBP12-rapamycin complex-associated protein;Mammalian target of rapamycin;mTOR;Mechanistic target of rapamycin;Rapamycin and FKBP12 target 1;Rapamycin target protein 1;MTOR;FRAP, FRAP1, FRAP2, RAFT1, RAPT1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-mTOR (S2481) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IEG-13 is a rabbit monoclonal antibody that recognizes serine/threonine-protein kinase mTOR phosphorylated at Ser2481 in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 288.9 kDa, and the supplier reports an observed band at about 289 kDa.
mTOR is a serine/threonine kinase that forms two complexes, mTORC1 and mTORC2, and serves as a central integrator of nutrient, growth factor and energy signals. mTORC1 drives protein, lipid and nucleotide synthesis through targets such as S6K and 4E-BP1 and suppresses autophagy. mTOR signaling is studied across cell growth, metabolism, aging, immunity and cancer.
| Target | Serine/threonine-protein kinase mTOR (gene MTOR; UniProt P42345, human) — phosphorylated at Ser2481 |
|---|---|
| Clone | IEG-13 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 288.9 kDa |
| Observed MW | 289 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): 293 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic phosphopeptide around Ser2481 of human mTOR.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places serine/threonine-protein kinase mTOR in the lysosome membrane, endoplasmic reticulum membrane and Golgi apparatus membrane. Tissue expression noted by UniProt (human): Expressed in numerous tissues, with highest levels in testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
