| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nucleolin; Protein C23; NCL |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-Nucleolin (T76) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29N32 is a rabbit monoclonal antibody that recognizes nucleolin (gene NCL) phosphorylated at Thr76 in human samples and is validated for Western blot and IHC. The predicted molecular weight is 76.6 kDa, and the supplier reports an observed band at about 100–110 kDa.
Nucleolin (NCL) is the most abundant protein of the nucleolus in growing cells. It binds chromatin, pre-ribosomal particles and RNA, loosens chromatin through its contact with histone H1, and supports rRNA transcription and ribosome assembly. Because it also appears on the cell surface of many proliferating cells, nucleolin is widely studied in ribosome biogenesis and cancer biology.
| Target | Nucleolin (gene NCL; UniProt P19338, human) — phosphorylated at Thr76 |
|---|---|
| Clone | 29N32 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 76.6 kDa |
| Observed MW | 100–110 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic phosphopeptide around Thr76 of human nucleolin.
Reactivity Notes
The supplier lists reactivity with human. UniProt places nucleolin in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
