| Field | Specification |
|---|---|
| Target | |
| Alternative names | Retinoblastoma-like protein 2; 130 kDa retinoblastoma-associated protein; p130; Retinoblastoma-related protein 2; RBR-2; pRb2; RBL2; RB2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-p130(T986) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 30R10 is a rabbit monoclonal antibody that recognizes retinoblastoma-like protein 2 (gene RBL2) phosphorylated at Thr986 in human and mouse samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 128.4 kDa, and the supplier reports an observed band at about 128 kDa.
RBL2 (p130) is a retinoblastoma family protein that represses E2F-driven genes, pairing mainly with E2F4 and E2F5 to keep cells in a quiescent state. It also binds cyclin A and cyclin E complexes and helps maintain heterochromatin, and its phosphorylation status is followed in cell cycle and tumor suppressor studies.
| Target | Retinoblastoma-like protein 2 (gene RBL2; UniProt Q08999, human) — phosphorylated at Thr986 |
|---|---|
| Clone | 30R10 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 128.4 kDa |
| Observed MW | 128 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic phosphopeptide around Thr986 of human p130.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places retinoblastoma-like protein 2 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
