| Field | Specification |
|---|---|
| Target | |
| Alternative names | Mitogen-activated protein kinase 14; MAP kinase 14; MAPK 14; Cytokine suppressive anti-inflammatory drug-binding protein; CSAID-binding protein; CSBP; MAP kinase MXI2; MAX-interacting protein 2; Mitogen-activated protein kinase p38 alpha; MAP kinase p38 alpha; Stress-activated protein kinase 2a; SAPK2a; MAPK14; CSBP1; CSBP2; CSPB1; MXI2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Phospho-p38 (T180) |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 31M76 is a rabbit monoclonal antibody that recognizes mitogen-activated protein kinase 14 (gene MAPK14) phosphorylated at Thr180 in human samples and is validated for Western blot and IHC. The predicted molecular weight is 41.3 kDa, and the supplier reports an observed band at about 41 kDa.
p38α (MAPK14) is the best-studied p38 MAP kinase and is expressed in most cells. Activated by MKK3 and MKK6 in response to inflammatory cytokines, LPS and cellular stress, it phosphorylates kinases such as MK2 and many transcription factors to control cytokine production, differentiation and cell death, making it a common target in inflammation research.
| Target | Mitogen-activated protein kinase 14 (gene MAPK14; UniProt Q16539, human) — phosphorylated at Thr180 |
|---|---|
| Clone | 31M76 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 41.3 kDa |
| Observed MW | 41 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Immunogen
Synthetic phosphopeptide around Thr180 of human p38.
Reactivity Notes
The supplier lists reactivity with human. UniProt places mitogen-activated protein kinase 14 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Brain, heart, placenta, pancreas and skeletal muscle. Because the antibody is phospho-specific, signal depends on the phosphorylation state of the sample; a phosphatase-treated lysate is a useful negative control.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Gui-zhou Zheng et al. (2022) Dioscin induces osteosarcoma cell apoptosis by upregulating ROS-mediated P38 MAPK signaling. Drug Development Research. 10.1002/ddr.22009
Shiqiang Wu et al. (2023) Dehydrocostus lactone (DHC) promotes osteoblastic differentiation and mineralization through p38/RUNX-2 signaling. Journal of Biochemical and Molecular Toxicology. 10.1002/jbt.23601
Xianglong Meng et al. (2024) Efficacy of Rhamnus utilis Decne. aqueous extract in mice with acute alcoholic liver injury and metabolomic study. Heliyon. 10.1016/j.heliyon.2024.e32523
Juan-juan Li et al. (2018) Tenacissoside H exerts an anti-inflammatory effect by regulating the nf-κb and p38 pathways in zebrafish. Fish & Shellfish Immunology. 10.1016/j.fsi.2018.09.032
Yan-Zi LI et al. (2018) Protective effects of extracts of Schisandra chinensis stems against acetaminophen-induced hepatotoxicity via regulation of MAPK and caspase-3 signaling pathways. Chinese Journal of Natural Medicines. 10.1016/S1875-5364(18)30110-9
