| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Red fluorescent protein eqFP611;GFP-like chromoprotein; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-PAK4 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ADFF-16; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, Flow (as provided in the source record). Boster Bio Anti-Phospho-PAK4 + PAK5 + PAK6 (S474 + S560 + S602) Monoclonal Antibody catalog # MP01723. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: PAK4 (Red fluorescent protein eqFP611).
- Antibody format: Monoclonal; clone ADFF-16; isotype Rabbit IgG.
- Host: Rabbit.
- PTM context: Phospho site information provided: S474,S560,S602.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
PAK4 is a commonly studied target in molecular and cellular biology. Functional context (as provided): Pigment protein. . Reported cellular localization context: Lateral cell membrane ; Single-pass type I membrane protein . Cell junction, tight junction . Colocalizes with CLDN7 at the lateral cell membrane and tight junction. . Tissue expression notes (as provided): Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .
Research relevance and current trends
- Research context keywords from the source record include: Actin,Actin, etc.,Apoptosis,Cancer,Cell Biology,Cell Cycle,Cytoskeleton,Cytoskeleton/ECM,Intracellular,Kinases,Kinases/Phosphatases,Microfilaments,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
- When phosphorylation is relevant, researchers frequently track stimulus-driven changes and compare phospho-state to total protein abundance.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate PAK4 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect PAK4 expression by Western blot in cell or tissue lysates, Quantify PAK4-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 15 kDa; calculated MW: 26053 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 15 kDa
- Cellular localization (provided): Lateral cell membrane ; Single-pass type I membrane protein . Cell junction, tight junction . Colocalizes with CLDN7 at the lateral cell membrane and tight junction. .
- Tissue details (provided): Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
